Toxicology International (Formerly Indian Journal of Toxicology)

Rajat Pratap Singh , Pradeep Kumar Singh , Ram Lakhan Singh

Abstract

A bacterial strain RMLRT03 with ability to decolorize textile dye Acid Orange dye was isolated from textile effluent contaminated soil of Tanda, Ambedkar Nagar, Uttar Pradesh (India). The decolorization studies were performed in Bushnell and Haas medium (BHM) amended with Acid Orange dye. The bacterial strain was identified as Staphylococcus hominis on the basis of 16S rDNA sequence. The bacterial strain exhibited good decolorization ability with glucose and yeast extract supplementation as cosubstrate in static conditions. The optimal condition for the decolorization of Acid Orange dye by Staphylococcus hominis RMLRT03 strain were at pH 7.0 and 35oC in 60 h of incubation. The bacterial strain could tolerate high concentrations of Acid Orange dye up to 600 mg l‑1. The high decolorizing activity under natural environmental conditions indicates that the bacterial strain has practical application in the treatment of dye containing wastewaters.

Keywords

16S rDNA, BHM, cosubstrate, decolorization, textile dye

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